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Briefly, after excision, the kidneys were placed in ice-cold isolation buffer (200 mM mannitol, 50 mM sucrose, 5 mM KH 2 PO 4 , 5 mM MOPS, 0.1% fatty acid free BSA, 1 mM EGTA, adjusted to pH 7.15 with KOH), homogenized with a motor-driven tightly fitting glass-Teflon Potter grinder operated at 1,600 rpm and centrifuged at 4 C for 3 min, at 1,100 g av

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During this period, typical weight loss ranges from 2 to 5 percent of baseline body weight

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Third, protein protects lean muscle mass