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Rosetta Comparative Modeling for Library Design: Engineering Alternative Inducer Specificity in a Transcription Factor

Chronic Dosing Protocol: AOD9604 administered intraperitoneally at 250 g/kg/day hGH administered at 1 mg/kg/day Treatment duration: 1428 days depending on cohort Daily administration via intraperitoneal injection Acute Testing Protocol: Single AOD9604 dose: 2 mg/kg 3-AR agonist BRL37344: 250 g/kg (for comparison) Indirect calorimetry measurements performed Measured Outcomes: Body weight, food intake, white adipose tissue (WAT) and brown adipose tissue (BAT) weights, plasma glycerol levels (indicating lipolysis), and 3-AR mRNA expression in adipose tissue