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After washing in PBS, horseradish peroxidase-conjugated goat anti-rabbit IgG (ab175470, Abcam) diluted 1:200 in 1% rabbit serum in PBS was applied, and the slides were incubated for 30 min at room temperature

Current evidence does not establish that GLP-1 medications directly cause bone loss, though the substantial weight reduction they produce may influence bone density through indirect mechanisms

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2000) and its inhibition (for instance by S-nitrosylation) may result in anti-inflammatory effects (Morris et al