The RTqPCR mixture consisted of 5 L of SYBR Green Master Mix (Yeasen, Shanghai, China), 1 L of upstream primer, 1 L of downstream primer (with a final concentration of 0.2 M for each primer), and 2 L of cDNA, with enzyme-free water added to a final volume of 10 L
Always check the specific batch configuration sheet to align your calculations precisely with your testing protocol
As a basic characteristic of diabetes, hyperglycemia function as the initiating injury factor of BBB leakage, and other injury factors induced by hyperglycemia further aggravate the damage of BBB, such as advanced glycation end-products, inflammation, oxidative stress, metalloproteinase, astrocyte apoptosis, microglia polarization to swallow astrocytic end-feet, nerve/glial antigen 2 (NG2-glia) proliferation, pericyte death, and lower endothelial tight junction coverage
Keep a structured water bottle nearby and drink consistently throughout the day rather than chugging large amounts during meals
10.1016/j.molmet.2020.101102 95 NauckM
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