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Intranasal delivery represents the most significant methodological advancement because it bypasses hepatic first-pass metabolism entirely, but technique sensitivity (nasal cavity anatomy, administration volume, head positioning post-dose) introduces reproducibility challenges that subcutaneous injection avoids

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10.7554/eLife.45807 Elife 21 BorrioneL.BelliniH.RazzaL.AvilaA.BaekenC.BremA.et al (2020)

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AK, SS, and HZ contributed to the visualization