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Given that SYNCRIP depletion reduced SIRT1 expression, we examined whether the SYNCRIP-SIRT1 axis contributes to the downstream regulation of HK2 and metabolic reprogramming

Using 5-chloromethylfluorescein diacetate (CMFDA), which has been reported to be a potent and irreversible GSTO1 inhibitor 22 , we optimized a gel-based binding assay that measures competitive inhibition of CMFDA binding to recombinant GSTO1 or endogenous GSTO1 in a soluble proteome (Supplementary Fig

The appropriate peptide concentration, at the recommended dose, brings the expected supportive effect

However, it may also be an indication of other factors or conditions, such as oxidative stress