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Then, prepared cell lysates were plated on a 96-well plate (Nunc) overnight at 4 C and incubated with 5% BSA PBST (NaCl 137 mM, KCl 2.7 mM, Na 2 HPO 4 10 mM, KH 2 PO 4 1.8 mM, 0.1% Tween 20) for 2 h at room temperature (RT)

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Anti-inflammatory mechanism Natural products in psoriasis have an anti-inflammatory effect by blocking important signaling pathways like NF-B and MAPK pathways, regulating the production of pro-inflammatory cytokines like TNF-, IL-6, IL-17, and IL-23, and controlling the activity of immune cells like T-helper 17 (Th17) cells

Bertoglio, K., Jill James, S., Deprey, L., Brule, N., & Hendren, R

doi: 10.1016/j.biomaterials.2010.06.059 26 GuoLHongDWangSZhangFTangFWuTet al

M., Laties, A