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Signalling from the receptor continues after it has been internalised, and internalisation appears to instruct the strength and type of signalling resulting from ligand binding

For example, a common starting dose is 0.25 mg

When it does occur, it is often linked to changes in blood sugar, dehydration, or low blood pressure

Fortunately, many of the most common side effects are mild to moderate and often improve as the body adjusts to treatment

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Three days later, the media was refreshed with media containing 1 g/ml puromycin in which they were kept throughout the assay