Reactivation procedure for oxidized e-GST Different hemolyzed blood samples (total blood, isolated erythrocytes or serum) and purified BSA approximately at the same concentration present in hemolyzed blood (23 M), were all incubated for 60 min at 37 C in a dry block thermostat with 1 mM final concentration of DTT and 0.01 M potassium phosphate buffer (pH 8.0)
Structural basis for the oligomerization-mediated regulation of NLRP3 inflammasome activation
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2019 Sep 3;11(9):2073