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But in a demanding setup, the difference can be easier to notice

The mice were raised in standard environment with a temperature of 23-25 C and a 12-hour light-dark cycle and provided food and water ad libitum

DP cells will slowly overflow in DMEM/F-12 (Dulbeccos Modified Eagle Medium/Nutrient Mixture F-12), 20% (v/v) fetal bovine serum (FBS) supplemented with 100 units/ml penicillin and 0.1 mg/ml streptomycin (Invitrogen, Carlsbad, CA, and Sigma, St

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doi: 10.1186/s10194-021-01271-1 10

doi: 10.1038/ni.3772 2 AgarwalS.YadavA.ChaturvediR