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The loss of pigmentation appears to be related to immunological destruction of melanocytes and nevus cells [64]

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Overall, these findings collectively suggest that melanocortin receptor activation potentially promotes neuronal and glial cell function by reducing amyloid pathology and ameliorating neuroinflammatory responses

(2) For adherent cells: wash 2 to 3 times with pre-cooled PBS at 4C, scrape off the cells with a cell scraper or treat the cells with EDTA solution so that the cells are no longer adhering very tightly to the wall, and blow down the cells with a pipette