DNA Constructs As shown in Figure S4B in Supplementary Material, pcDNA3.1-Flag-GSTP, pcDNA3.1-Flag-HMGB1, pcDNA3.1-Flag-GSTM, six GSTP phosphorylation mutants including pcDNA3.1-Flag-GSTP-S42A (in which serine-42 was mutated to alanine, a S42 non-phosphorylatable mutant), pcDNA3.1-Flag-GSTP-S42D (in which serine-42 was mutated to aspartate, a Ser42 constant-phosphomimetic mutant), pcDNA3.1-Flag-GSTP-S184A (in which serine-184 was mutated to alanine, a Ser184 non-phosphorylatable mutant), pcDNA3.1-Flag-GSTP-S184D (in which serine-184 was mutated to aspartate, a Ser184 constant-phosphomimetic mutant), pcDNA3.1-Flag-GSTP-Y198F (in which tyrosine-198 was mutated to phenylalanine, a Tyr198 non-phosphorylatable mutant) and pcDNA3.1-Flag-GSTP-Y198D (in which tyrosine-198 was mutated to aspartate, a Tyr198 constant-phosphomimetic mutant), pET28a-HMGB1, pET28a-GSTP(WT), pET28a-GSTP(S184A), pET28a-GSTP(S184D) were constructed by using molecular cloning technology
Once-weekly administration of a long-acting fibroblast growth factor 21 analogue modulates lipids, bone turnover markers, blood pressure and body weight differently in obese people with hypertriglyceridaemia and in non-human primates
Changes in Redox Signaling in the Skeletal Muscle with Aging
With potential benefits in tanning, vitiligo, and solar urticaria research, these vials pave the way for innovative studies aimed at advancing our understanding of melanin-related processes
10.1016/j.ejphar.2024.176706 Eur
Han, C., Lim, K., Xu, L., Li, G
