Following 3 h of drug treatments, 5 M of the cell-permeable, ROS-sensitive dye CellROX Deep Red (Invitrogen, Stockholm, Sweden) was added to each sample and incubated at 37 C for 30 min
To validate the effectiveness of A-RDN, protein levels of calcitonin gene-related peptide (CGRP), which is the dominant neurotransmitter for afferent renal nerves [37, 38], and tyrosine hydroxylase (TH), a marker for efferent sympathetic fibers in the renal pelvis were quantified
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The body has learned to access stored fuel