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CYP3A4, CYP3A5 and P-glycoprotein One of the most important determinants of Tac PK is the first-pass effect, regulated by Cytochrome P450 enzymes CYP3A4 and CYP3A5 as well as the P-glycoprotein (P-gp), which are predominantly expressed in the liver and gut, but can also been found in other tissues

Cells were spun down at 3000 g for 5 min, the supernatant was discarded, the pellet was resuspended in 30 mL cold H 2 O and spun down as before described, supernatant was discarded

Najmi et al., 2022

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