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2 Materials and methods 2.1 Experimental animals Male C57BL/6J mice [specific-pathogen-free (SPF) grade], aged 810 weeks and weighing 2025 g, were obtained from the Laboratory Animal Center of Ningxia Medical University

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Preclinical work adds two more data points: in rats, daily subcutaneous ipamorelin dose-dependently increased longitudinal bone-growth rate without changing total IGF-1 or bone-turnover markers, suggesting a partly local, GH-pulse-driven skeletal effect rather than a purely IGF-1-mediated one [15]

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