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doi: 10.1055/s20071003242 201 DobnerPRKawasakiESYuLYBancroftFC

The most straightforward method consists of culturing suspended cells in a nutrient medium, allowing their natural tendency to aggregate to form structured 3D cell clusters, known as spheroids [245]

When ROS levels exceed the metabolic capacity of active glutathione, cell damage, thrombosis, and acute hemolytic anemia may occur

Glutathione might just be the secret ingredient you need

Notably, heme oxygenase 1 (HO1), which has a dual role in regulating ferroptosis, oxidizes cellular heme to carbon monoxide, ferrous iron, and biliverdin [25]

While effective, it can produce some complications