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Following primary antibody incubation, slides were washed three times with PBS (5 min each) and then incubated with enzyme-conjugated goat anti-mouse or anti-rabbit IgG polymer for 30 min at room temperature

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Both microvascular and macrovascular endothelial cells express GLUT1 and early endothelial dysfunction in T2D is thought to be a driver of future cardiovascular disease [112]

The reconstituted solution must be refrigerated immediately and used within the timeframe specified by your pharmacy