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This step identifies contraindications before injection

Despite providing important mechanistic insights, require further validation in randomized clinical trials (RCTs)

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The supernatant was transferred to LCMS vials, and the pellet was lysed by 20 mM NaOH and protein levels were detected for normalization

( Mutation detection and gene fusion analysis were performed using the customized panels QIASeq targeted DNA Panel for Solid Tumors (Qiagen) and QIAseq targeted RNAscan Panel (Qiagen)

plasma IL-1, TNF-, and IL-6 concentrations positively correlate with the extent of LDH and intensity of low back pain [21]