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The final predicted expression of both CBS OPT and L2HGDH OPT were MYPYDVPDYALQISCBS OPT (or L2HGDH OPT )DYKDDDDKKSRGPGRATGAAANSAVDGTAGPGSTGSRVDGSGEGRGSLLTCGDVEENPGP (with the features: HA tag, amino acid linker 1, CBS OPT (or L2HGDH OPT ), FLAG, amino acid linker 2, T2A self-cleavage site, in the order of N to C terminal) (#230088 and #230089, Addgene)
Au et al
doi: 10.1007/BF01141363
Methods: This is a prospective, investigator-initiated, proof-of-concept pilot study: Evaluating LIver Stiffness and steatosis as a TM in patients with AUD (ELISA)
Goto S, Iida T, Cho S, Oka M, Kohno S, Kondo T
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