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Total RNA from treated senescent ATDC5 cells was extracted with Trizol (Thermo Fisher Scientific), and RNA concentrations were determined by spectrophotometry (Nanodrop 2000

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Neurobion Forte RF Injection should only be administered by a trained healthcare professional

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After each blood sample was taken, the catheter was flushed with heparinized lactated Ringer's solution and a volume of heparinized lactated Ringer's equal to the volume of blood taken was injected (to maintain total blood volume)