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Neuron 77 , 886898 (2013)

An intracellular antioxidant called glutathione (GSH) is produced from glutamate, cysteine, and glycine (174)

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GPX4 genetic silencing in vitro The lentiviral plasmids, either non-targeted vector (pLKO.1-shNT) or pLKO.1-shGPX4 plasmid DNAs (Sigma-Aldrich) were transfected along with packaging and envelop plasmids psPAX2 and pMD2.G into HEK293T cells