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Once in the brain, caffeine blocks adenosine receptors, which reduces the sense of tiredness and supports sustained concentration

For the kinetic analysis, assays were carried out with -glutamyl- S -allyl-L-cysteine concentrations ranging from 12.5 to 1000 M for AsGGT1, 0.58 mM for AsGGT2, and 125 mM for AsGGT3

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The MAPK phosphatases have a similar catalytic site (VHCX5R(S/T)) (74, 75), with cysteine being essential for MAPK dephosphorylation