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Lut and Bc showed characteristic peaks of 963.27 and 964.23 cm 1 , receptively, which represent the deformation vibration of trans-conjugated alkene in the polyene chain 63,66,67 , this peak disappeared or may be overlapped with the appearance of the C-H deformation vibration band of T80 after formulated as nanodispersion at 947.84, 949.77, and 947.84 of T80, Nano-Lut, and Nano-Bc, respectively
The cells were blocked using 1% BSA for 1 h to eliminate non-specific binding
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4 Further to this, metabolic disorders, for example diabetes and obesity, have been shown to negatively alter the bone microenvironment and compromise bone quality