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The HBEC cells were cultured in K-SFM, and the other cells were cultured in DMEM essential medium with 10% fetal calf serum at 37 C in a humidified incubator in an atmosphere of 95% air and 5% CO 2

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Long-term studies show significant weight regain over 6-12 months of discontinuation, but a structured short break with proper weight maintenance strategies typically results in 3-8 pounds of regain, much of which is water weight and glycogen restoration that reverses quickly upon restarting medication

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Body mass index and mortality in heart failure: a meta-analysis