Treatment of Arabidopsis seedlings with 5 mM H 2 O 2 increased GCL activity and resulted in a shift from the less active reduced form to the more active oxidized form, as determined by activity assays and immunoblot analysis (Hicks et al., Conservation of the disulfide linkage that mediates the monomer/dimer transition of GCL occurs across the plant kingdom (Gromes et al., Nicotiana tabacum ), Agrobacterium tumefaciens (a-proteobacteria), and Xanthomonas campestris (-proteobacteria) showed that the two proteobacterial GCL remain monomeric during redox changes, even though the disulfide is retained
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Hickman MJ, Petti AA, Ho-Shing O, Silverman SJ, McIsaac RS, Lee TA, Botstein D
W., Peck, B., and Schulze, A
Role of oxidative metabolism in the localization of plant viruses
The word antioxidant often brings to mind vitamins C or E, but actually, glutathione (GSH) is the most powerful and abundant antioxidant in the human body