Postfixative was removed and cells were washed (five times for 1 min each) with 0.1 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and then washed (five times for 1 min each) with cold double-distilled H 2 O (ddH 2 O) on ice
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It binds to various toxins, making them more water-soluble so the body can move them out through the liver and kidneys
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When used in combination with lifestyle modifications such as exercise and diet, lipotropic compounds may promote fat and weight loss
However, while increasing structural complexity can amplify antimicrobial potency, it also raises challenges related to reproducibility, biosafety, and the lack of standardised toxicological evaluation
