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The butylated samples were dried again under nitrogen and reconstituted in 100 L of ACN:H 2 O (1:1) for 30 min, and vortexed for another 20 s before being aspirated into a pulled vial insert for high-performance liquid chromatography tandem mass spectrometry (HPLCMS/MS) analysis (Smith & Matern, 2010)

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The mean BMI was 34.0 kg/m2

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