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The next layer of the TTDS is the biological core, which historically formed the heart of activated sludge systems

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The linear increase in absorbance at 412 nm over time was monitored using a microplate reader (SpectraMax M3 Molecular Devices, United States)

Deeply rooted affective responses, often described as the yuck factor and characterized by instinctive disgust and fear of contamination, continue to impede acceptance, particularly for potable reuse involving direct human contact

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