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The sulfur-containing thiol group in cysteines side chain is key to its properties, enabling the formation of disulfide bridges between two peptide chains (as with insulin) or loop formation within a single chain, impacting the final protein structure

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As the main methyl donor in cells, SAM also mediates a variety of methylation reactions, aside from DNA methylation, including histone, RNA, and some protein amino acid residue methylation (Teperino et al., 2010)

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The effect depends on dose, delivery method, and consistency