For the promoter assay, the fragment (3000 bp to +1000 bp) of human PHGDH, PSAT1, PSPH and SLC1A4 promoters, containing Np63 putative binding sites, was inserted into the Gluc-On promoter reporter vector (pEZX-PG04, GeneCopoeia, Guangzhou, China) and designated as PHGDH-Gluc, PSAT1-Gluc, PSPH-Gluc, SLC1A4-Gluc, respectively
found that DEAD-box helicase 17 acted together with B-cell lymphoma 6 to inhibit Drp1 in cardiomyocytes to regulate cardiomyocyte homeostasis
All HEDS assays were started by the addition of enzyme
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NAC
H 2 O 2 (30%, 7.5 mL) was slowly added, with continuous stirring, for 30 min at ambient temperature