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In a rat model of focal cerebral ischemia induced by a 90 min occlusion of the middle cerebral artery (MCAO), the researchers compared the effects of a five-day pretreatment with ceftriaxone (CEF) or NAC to those of ischemic and chemical preconditioning protocols

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BrdU-positive MCs were visualized using fluorescence microscopy (see below) and quantified from at least 20 randomly positioned micrographs