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The specimen was visualized with a Titan Krios electron microscope (FEI) operating at 300 kV accelerating voltage, at a nominal magnification of 29,000X using a K2 Summit direct electron detector (Gatan, Inc.) in counting mode, corresponding to a pixel size of 1.0 on the specimen level

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Glutathione is synthesized in two sequential enzymatic reactions: glutamate cysteine ligase (GCL) catalyzes the first, rate-limiting step, and glutathione synthetase couples -glutamylcysteine to glycine in the last step [13]