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doi: 10.1136/bmj.j4849 10 CooperTCSimmonsEBWebbKBurnsJLKushnerRF

[36] [37] GST-tags and the GST pull-down assay [edit] GST can be added to a protein of interest to purify it from solution in a process known as a pull-down assay

The affected enzymes, ERK1/2, along with their downstream targets, including c-Fos, c-Jun, and Egr-1, are believed to play significant roles in cell growth, migration, and angiogenesis, which is the development of new blood vessels

doi: 10.1016/j.bbamcr.2010.10.007

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Isolation and culture of primary mouse melanocytes Primary mouse MCs were isolated from the epidermis of newborn wt mice (C57Bl6/N, male and female) and maintained up to passage 3 as previously described (Mescher et al, 2017)