R&D Systems, Minneapolis, MN) in RPMI-1640 (Gibco, Detroit, MI) medium supplemented with 2% penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco) and 5% pooled normal human heat-inactivated and sterile-filtered AB serum (Innovative Research, Novi, MI) for 7 days, with a media change on day 5 with fresh M-CSF (10 ng/mL)
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All procedures and testing were performed in a blinded manner
If any food reintroductions led to symptoms such as diarrhea or pain, they were excluded
GRX-catalyzed deglutathionylation through a monothiol mechanism is also likely part of the catalytic cycle of Arabidopsis MSRB1 (At1g53670) whereas MSRB2 (At4g21860) is regenerated through a TRX-dependent dithiol mechanism (Tarrago et al., 2009)
Bottom line: Support your bodys natural elimination pathways with water and fibre, start your glutathione journey slowly, and always consult a professional if you have concerns about existing medical conditions
