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The combined administration of GH-releasing peptide-2 (GHRP-2), TRH and GnRH to men with prolonged critical illness evokes superior endocrine and metabolic effects compared to treatment with GHRP-2 alone

Even though opiates were suspected to induce GH secretion via a direct action in the hypothalamus rather than through the pituitary gland and were not thought to be GHRH itself, the possibility was considered that these natural peptides might release GH through a hypothalamic and pituitary mechanism

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The leaves were washed with distilled water, air-dried, and then dried in a laboratory oven (DIGITAL TT 9083, Techmel & Techmel USA) at a regulated temperature of 40C