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Human and mice intestinal organoids cultivated in expansion and refined media were incubated with 5-ethynyl-2deoxyuridine (EdU) at a final concentration of 10 M for 30 min at 37 C and then fixed with 4% PFA for 2 h at room temperature

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Moriguchi S, Ishizuka T, Yabuki Y, Shioda N, Sasaki Y, Tagashira H, et al

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The total recurrence rate was 36.84%, and 57.14% of that percentage was associated with metastasis