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Once this is done, take your opposite hand that holds the syringe and needle and place the needle into the skin just beneath your bunched fingers

Frias JP, et al

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Step 3: Inject with Minimal Discomfort To make the injection as painless as possible, start by cleaning the chosen spot with an alcohol wipe and letting it air-dry completely

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Wnt3a, -catenin, PCNA, Cyclin D1, and VEGF increased in dorsal skin on day 13, as detected by WB