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Samples were separated using a Kinetex 2.6 m XB-C18 100 , 100 2.1 mm LC Column (Phenomenex) with a constant flow rate of 250 l/min following a flow gradient starting at 70% Mobile Phase B (ACN + 0.5% Acetic acid, AA) then going up to 99% for 5 min before re-equilibration at 70% for 5 min

Observe minutely and record any untoward event to the provider

The majority of Mn, primarily as Mn 2+ , carried by globulin and albumin in the serum, enters the cell via transmembrane transporters such as ZIP8, ZIP14, and DMT1

Ferroptosis: principles and significance in health and disease

Proton pump inhibitors, like omeprazole, can also impair vitamin B12 absorption by reducing stomach acid production

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