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Coronal sections (250 mm) were cut using the Leica VT1000S Vibratome and then incubated in oxygenated aCSF (3234 C) for at least 1 h before recordings

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12 The matched factors included demographics (age at index event, sex, race, and ethnicity), diagnoses (chronic kidney disease, obstructive sleep apnea, and nicotine dependence), medications (amiodarone, dipeptidyl peptidase 4 (DPP-4) inhibitors, insulin and insulin analogs, sulfonylureas, biguanides, sodium-glucose co-transporter 2 (SGLT2) inhibitors, and thiazolidinediones), and clinical laboratory values like body mass index and hemoglobin A1c levels

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