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[DOI] [PubMed] [Google Scholar] 107.Misra A.L., Woods L.A

(1997) 273:G717

To minimize potential contamination from minor neuronal signals, an arbitrary boundary was defined around each astrocyte based on its characteristic morphology, including visible processes and branches

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Following three washing steps with PBS, cells were permeabilized with 0.1% Triton for 20 min and blocked with 2% BSA in PBS-Tween (0.5%) at room temperature for at least 1 h

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