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Age-matched healthy female controls were recruited

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Following another 24-h incubation, cells were washed with PBS and bioluminescence was measured using the Nano-Glo Live Cell Assay System (Promega), according to the manufacturers instructions, using a Tecan Spark plate reader

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For non-reducing SDS-PAGE, 2 g of protein was mixed with 3 L of 4 Bolt LDS Sample Buffer (Invitrogen) and Milli-Q water to a total volume of 12 L