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Carnitine concentration determination After the feeding trial, the whole liver and parts of muscle of 4 fishes collected from each group were weighted and homogenized by pure-water (1:10, w/v) by using a drill-driven Teflon glass homogenizer (Scientz Biotec, China) with 34 strokes

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doi: 10.3390/ijms22189987 334 SegreJ

Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)

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