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Generation of cAMP was determined in 100 l containing: 10 mM HEPES, 12 mM MgCl 2 , 60 mM NaCl, 1.2 mM EDTA, 1.2% w/v BSA, 5% DMSO, 480 M ATP, 2 mM IBMX, 10 M GTP, 20 g membrane and ligands as indicated
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2 Pathological mechanisms of heat stress-induced sperm damage 2.1 Spermatogenic microenvironment disruption and resultant failure Spermatogenesis in mammals constitutes a highly intricate and precisely orchestrated process of cellular differentiation, the fidelity of which depends upon the stability of the testicular spermatogenic microenvironment
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