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1.1 Protein extraction (1) Prepare solution: melt protein lysate at room temperature RIPA , add Protease Phosphatase Inhibitor mixture (50X) to give a final concentration of 1X, mix well and place on ice immediately

Determination of intracellular cysteine The cysteine content in the cells was determined by the method of Cotgreave and Moldeus (1986) with slight modifications

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International journal of sports medicine, 16(2), 114116

Am J Physiol Heart Circ Physiol 2007