10.1038/s41419-019-1564-7 Cell Death Dis
The solution is no longer bacteriostatic
Consistency is Key : To achieve the best results, its important to take these supplements consistently over time
[Rotruck first established the selenium dependence of the GSH-peroxidase that relied on glucose-6-phosphate dehydrogenase-GSH-reductasedriven recycling of GSH to protect erythrocytes from oxidation (311, 312): in erythrocytes from selenium-deficient rats, glucose-mediated reduction of GSH had no protective effect against oxidation.] In fact, in studies of hydrogen peroxide metabolism in cultured cells from GPx-1-deficient and wild-type mice, exposure to hydrogen peroxide led to a transient increase in oxidized GSH in wild-type cells, whereas GSSG did not fluctuate in GPx-1-deficient cells (229)
Therefore, preventing microbial contamination keeps the solution usable during its shelf life
The two main approaches are subcutaneous injection (under the skin, typically in the abdominal fat pad) and intramuscular injection (into a muscle belly)