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Advancements in mass spectrometry (MS)-based proteomics combined with bioinformatics 22 have emerged as powerful tools for characterising these molecular changes 21

Table 1 shows the detailed inclusion, exclusion, and removal criteria

Conformational energy analysis suggested that PNC-27 may form relatively stable complexes with HDM-2, potentially allowing the leader sequence to align in a way that minimizes interference with the core binding interaction, although its role in the overall pore structure cannot be definitively confirmed

Conventional extraction methods mainly rely on Soxhlet, maceration, and infusion

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