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Diabetologia 59:21562165 Northridge DB, Jardine AG, Alabaster CT et al (1989) Effects of UK 69 578: a novel atriopeptidase inhibitor

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Total RNA was isolated with the TRIzol reagent and centrifuged at 12,000 g for 15 min at 25C following the addition of chloroform

they can however be maintained in culture after 14 days of maturation for an additional 2 weeks in Williams E Medium

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