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For pyruvate tolerance test, glycerol tolerance test and CL316432 loading test, 16-hr fasted mice were administered intraperitoneally with 1 g/kg sodium pyruvate, 0.5 g/kg glycerol and 0.1 mg/kg CL316432 (Tocris Bioscience, Bristol, UK), respectively and blood glucose levels were monitored at the indicated time points