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Krebs Hepes buffer contains 99 mM NaCl, 4.69 mM KCl, 2.5 mM CaCl 2 2H 2 O, 1.2 mM MgSO 4 7H 2 O, 25 mM NaHCO 3 , 1.03 mM KH 2 PO 4 , 5.6 mM d-(+)-glucose, and 20 mM Na-Hepes

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