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To address copper-dependent toxicity in WD in human liver cells, we reanalysed the RNA sequencing data (GSE107323) [2], validated gene expression signatures and functionally addressed our findings in the HepG2 ATP7B knockout (ATP7B-KO) model [7]

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Lack of long-term safety data Risk of infection or dosing errors with injections Overdosing of copper , especially in those who may ingest or inject GHK-Cu as a supplement

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